#/usr/bin/perl -w
use strict; my ($len,$total)=(0,0); my @x; while(<>){ if(/^\>\@/){ if($len>0){ $total+=$len; push @x,$len; } $len=0; } else{ s/\s//g; $len+=length($_); } } if ($len>0){ $total+=$len; push @x,$len; } @x=sort{$b<=>$a} @x; my ($count,$half)=(0,0); for (my $j=0;$j<@x;$j++){ $count+=$x$j; if (($count>=$total/2)&&($half==0)){ print "N50: $x$j\n"; $half=$x$j }elsif ($count>=$total*0.9){ print "N90: $x$j\n"; exit; }
N50 N90 calculation using Perl code
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